From 40a7c0b1e7189f30e33d12ed514321f2797ab156 Mon Sep 17 00:00:00 2001 From: Juan Santos <39328739+Ingefast@users.noreply.github.com> Date: Thu, 10 Nov 2022 10:58:22 +0100 Subject: [PATCH] Update README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 9817d23..e5bc44b 100644 --- a/README.md +++ b/README.md @@ -195,7 +195,7 @@ The processed data provided so far opens a wide range of analytical possibilitie Two common basic analyses are presented here as examples. ## 1. Checking data structure and replicability in the data set. Multivariate analysis and correlogram. -With **sRNA.correlogram_plotter.r** it is possible to analyse the table **total_table.size_24.genes.txt** to plot the correlation between particular sRNA expression values across all the samples. A scatterplot in the lower diagonal panel is presented and Pearson correlation coefficients in the upper panel (Figure 2B). The script **sRNA.ordination_analyser.r** performs a multivariate analysis using the same input table to evaluate the similarities between samples. By default the samples are ordinated with Nonmetric multidimensional scaling (NMDS) as implemented in the R library vegan (Figure 2C). Principal component analysis (PCA) and redundancy analysis (RDA) are also available as alternative ordination approaches. +With **sRNA.correlogram_plotter.r** it is possible to analyse the table **total_table.size_24.genes.txt** to plot the correlation between particular sRNA expression values across all the samples. This is a good way to check for deviant samples and assess replicability. A scatterplot in the lower diagonal panel is presented and Pearson correlation coefficients in the upper panel (Figure 2B). The script **sRNA.ordination_analyser.r** performs a multivariate analysis using the same input table to evaluate the similarities between samples. By default the samples are ordinated with Nonmetric multidimensional scaling (NMDS) as implemented in the R library vegan (Figure 2C). Principal component analysis (PCA) and redundancy analysis (RDA) are also available as alternative ordination approaches. ![This is an image](/images/figure1.png)