diff --git a/README.md b/README.md index d8c5964..d6ca7e4 100644 --- a/README.md +++ b/README.md @@ -71,8 +71,6 @@ working_dir="/media/diskb/nicolas_tmp/REPLICATES_TOTAL" #this defines the samples to be analysed (dir names) sample_list="mutant_rep1 mutant_rep2 wt_rep1 wt_rep2"; -#sample_list="test_rep1"; - ``` If removing structural RNAs is wanted before downstream processing, a fasta file with the selected structural RNAs (including e.g. pre-tRNA, snoRNA, snRNA, rRNA) has to be prepared (with e.g. bedtools getfasta) and bowtie-indexed accordingly. Otherwise the pipeline can easily be modified skipping this step.