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openimadis_downloader.java
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openimadis_downloader.java
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package plugins.perrine.openimadisutilities;
import java.awt.Rectangle;
import java.io.IOException;
import java.util.concurrent.Callable;
import java.util.concurrent.ExecutionException;
import java.util.concurrent.Future;
import com.strandgenomics.imaging.iclient.ImageSpace;
import com.strandgenomics.imaging.iclient.ImageSpaceObject;
import com.strandgenomics.imaging.iclient.PixelMetaData;
import com.strandgenomics.imaging.iclient.RecordBuilder;
import com.strandgenomics.imaging.icore.Dimension;
import com.strandgenomics.imaging.icore.IPixelData;
import com.strandgenomics.imaging.icore.ImageType;
import com.strandgenomics.imaging.icore.Site;
import com.strandgenomics.imaging.icore.SourceFormat;
import com.strandgenomics.imaging.icore.image.PixelArray;
import com.strandgenomics.imaging.icore.image.PixelDepth;
import com.strandgenomics.imaging.iclient.Project;
import com.strandgenomics.imaging.iclient.impl.ws.ispace.Record;
import com.strandgenomics.imaging.icore.Channel;
import icy.image.IcyBufferedImage;
import icy.sequence.Sequence;
import icy.system.thread.ThreadUtil;
import icy.type.DataType;
import plugins.adufour.blocks.lang.Block;
import plugins.adufour.blocks.util.VarList;
import plugins.adufour.ezplug.EzPlug;
import plugins.adufour.ezplug.EzVar;
import plugins.adufour.ezplug.EzVarInteger;
import plugins.adufour.ezplug.EzVarListener;
import plugins.adufour.vars.lang.VarSequence;
public class openimadis_downloader extends EzPlug implements Block{
/**
* TODO put an option and query(several GUID selection) OR GUID. For now one guid to be compaticble with cluster processing.
* @author Perrine
*
*/
EzVarInteger guid=new EzVarInteger( "GUIDtoprocess");
EzVarInteger cropx1=new EzVarInteger( "Crop in X from ");
EzVarInteger cropx2=new EzVarInteger( "To: ");
EzVarInteger cropy1=new EzVarInteger( "Crop in Y from ");
EzVarInteger cropy2=new EzVarInteger( "To: ");
VarSequence outputsequence=new VarSequence("Downloaded Sequence", null);
@Override
public void clean() {
// TODO Auto-generated method stub
}
@Override
protected void execute() {
// TODO Auto-generated method stub
if (isHeadLess()){
//nothing set in block-> get full size
if (cropx2.getValue()==0||cropy2.getValue()==0){
ImageSpace ispace = ImageSpaceObject.getConnectionManager();
if (ispace==null){
}
else{
com.strandgenomics.imaging.iclient.Record record = ispace.findRecordForGUID(guid.getValue());
if (record==null){
cropx2.setValue(0);
cropy2.setValue(0);
}
else{
cropx2.setValue(record.getImageWidth()-1);
cropy2.setValue(record.getImageHeight()-1);
}
}}}
outputsequence.setValue(downloadSequence(guid.getValue(), cropx1.getValue(), cropy1.getValue(), cropx2.getValue()-cropx1.getValue()+1, cropy2.getValue()-cropy1.getValue()+1));
if (isHeadLess()){
}else
addSequence(outputsequence.getValue());
}
private Sequence downloadSequence(final Integer myguid, final Integer offsetX,final Integer offsetY,final Integer sizeX,final Integer sizeY) {
// TODO Auto-generated method stub
Future<Sequence> seqresult=ThreadUtil.bgRun(new Callable<Sequence>(){
@Override
public Sequence call(){
ImageSpace ispace = ImageSpaceObject.getConnectionManager();
com.strandgenomics.imaging.iclient.Record record = ispace.findRecordForGUID(myguid);
if (record==null)
{
String user=ispace.getUser();
System.out.println("this record id does not exist or you do not have access with your account, currently: "+user);
return null ;
}
System.out.println("This record contains "+record.getFrameCount()+" time points "+record.getSliceCount()+" z planes and comes from Project: "+record.getParentProject()+ "\n Total Size is "+(record.getSliceCount()*record.getFrameCount()*record.getChannelCount()*record.getImageHeight()*record.getImageWidth()*record.getPixelDepth().getByteSize())/1000000+" MBytes.");
Sequence seq = new Sequence();
double averagevalue=0.0;
float[] values= new float[record.getImageWidth()*record.getImageHeight()];
int nChannel=record.getChannelCount();
int nFrames=record.getFrameCount();
int nSlices=record.getSliceCount();
Rectangle myRoi=new Rectangle(offsetX,offsetY,sizeX,sizeY);
try{
for(int time = 0;time<nFrames;time++)
{
double elapsedtime=0;
for(int slice = 0;slice<nSlices;slice++)
{
IcyBufferedImage img = new IcyBufferedImage((int)myRoi.getWidth(), (int) myRoi.getHeight(), nChannel, getDataType(record.getPixelDepth()));
for(int channel = 0;channel<nChannel;channel++)
{
if (!isHeadLess())
getUI().setProgressBarValue((double)(channel+slice*nChannel+time*nSlices*nChannel)/(double)(nChannel*nFrames*nSlices));
IPixelData pixelData = record.getPixelData(new Dimension(time, slice, channel, 0));
System.out.println(String.format("% ,.1f",100*(double)(channel+slice*nChannel+time*nSlices*nChannel)/(double)(nChannel*nFrames*nSlices))+ " % downloaded");
//Object values = pixelData.getRawData().getPixelArray(); //old
PixelArray pa;
pa = (PixelArray) pixelData.getRawData(myRoi);
for (int i = 0; i < myRoi.getHeight()*myRoi.getWidth(); i++) {//n
values[i] = (float) pa.getPixelValue(i);//n
}//n
img.setDataXY(channel, values);//old
elapsedtime=pixelData.getElapsedTime();
}
seq.addImage(time, img);
}
averagevalue=elapsedtime;
}
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}//n
if (record.getFrameCount()>1)
averagevalue=averagevalue/(record.getFrameCount()-1); //in ICY metadata indicated are the time interval (duration of the movie)
seq.setName(record.getSourceFilename()+"_guid_"+myguid);
//Set Meta Data (minimum: pixel size and time increment, computed in average: to be checked: image based metadata in ICY)
double physicalSizeX=record.getPixelSizeAlongXAxis(); //in um
double physicalSizeY=record.getPixelSizeAlongYAxis();
double physicalSizeZ=record.getPixelSizeAlongZAxis();
if (averagevalue>0)
seq.setTimeInterval(averagevalue); //in seconds
if (physicalSizeX>0)
seq.setPixelSizeX(physicalSizeX);
if (physicalSizeY>0)
seq.setPixelSizeY(physicalSizeY);
if (physicalSizeZ>0)
seq.setPixelSizeZ(physicalSizeZ);
return seq;
}});
try {
return seqresult.get();
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
return null;
} catch (ExecutionException e) {
// TODO Auto-generated catch block
e.printStackTrace();
return null;
}
}
@Override
protected void initialize() {
addEzComponent(guid);
addEzComponent(cropx1);
addEzComponent(cropx2);
addEzComponent(cropy1);
addEzComponent(cropy2);
guid.addVarChangeListener(new EzVarListener<Integer>()
{
@Override
public void variableChanged(EzVar<Integer> source, Integer newValue)
{
if ((newValue!=null)&&(newValue!=0)){
// we get the metadata of this file
ImageSpace ispace = ImageSpaceObject.getConnectionManager();
if (ispace==null){
}
else{
com.strandgenomics.imaging.iclient.Record record = ispace.findRecordForGUID(newValue);
if (record==null){
cropx2.setValue(0);
cropy2.setValue(0);
}
else{
cropx2.setValue(record.getImageWidth()-1);
cropy2.setValue(record.getImageHeight()-1);
}
}
}
}
});
}
@Override
public void declareInput(VarList inputMap) {
// TODO Auto-generated method stub
inputMap.add("STRANDGUID", guid.getVariable());
inputMap.add("cropx1", cropx1.getVariable());
inputMap.add("cropx2",cropx2.getVariable());
inputMap.add("cropy1", cropy1.getVariable());
inputMap.add("cropy2", cropy2.getVariable());
}
@Override
public void declareOutput(VarList outputMap) {
outputMap.add("SEQUENCEdownloaded", outputsequence);
}
private DataType getDataType(PixelDepth pixelDepth)
{
if(pixelDepth == PixelDepth.BYTE)
return DataType.UBYTE;
if(pixelDepth == PixelDepth.SHORT)
return DataType.USHORT;
if(pixelDepth == PixelDepth.INT)
return DataType.UINT;
throw new IllegalArgumentException("unknown data type");
}
}