Skip to content

Latest commit

 

History

History
66 lines (50 loc) · 1.8 KB

README.md

File metadata and controls

66 lines (50 loc) · 1.8 KB
Raw

edar-xedar-expression-analysis

R scripts for analyzing Edar and Xedar gene expression data in mouse and rat tail development

Gene Expression Analysis Scripts

This repository contains R scripts for analyzing gene expression data from the study "Differential Expression of Edar and Xedar During Mouse and Rat Tail Appendage Development".

Overview

The scripts perform statistical analyses of Edar and Xedar gene expression data, including:

  • Data quality control and normalization
  • Two-way ANOVA with post-hoc tests
  • Correlation analysis
  • Polynomial regression modeling
  • Data visualization

Requirements

The following R packages are required:

  • stats
  • ggplot2
  • car
  • nlme

##Install using:

"install.packages(c("stats", "ggplot2", "car", "nlme"))"

##Input Data Format
The scripts expect a CSV file named "edar_xedar_expression.csv" with the following columns:

day: developmental day (numeric)
species: mouse or rat (factor)
edar: Edar expression value (numeric)
xedar: Xedar expression value (numeric)
actin: β-actin expression value (numeric)

##Example
day,species,edar,xedar,actin
11,mouse,55.81,1176.26,1.02
12,rat,90.44,1833.53,0.98

##Usage
Place your data file in the working directory
Run the analysis:
source("expression_analysis.R")

##Output
The script generates:

Statistical test results (saved in analysis_results.RData)
Expression pattern plots (saved as PDF)
R-squared values for polynomial models
ANOVA results and correlation coefficients

##Citation
If you use these scripts, please cite:
Wisniewski SA (2024) Differential Expression of Edar and Xedar During Mouse and Rat Tail Appendage Development
Figshare repository DOI: 10.6084/m9.figshare.27927177


##Contact
For questions or issues, please contact:
sa.wisniewski@sci4biz.edu.pl

##License
This code is available under the CC-BY 4.0 license.