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bug: Yarrowia lipolytica should have complex I for respiratory chain #15

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feiranl opened this issue Sep 18, 2020 · 3 comments
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@feiranl
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feiranl commented Sep 18, 2020

Yarrowia lipolytica should have complex I for respiratory chain, not the alternative internal NADH:ubiquinone oxidoreductase(NDI1 in S. cervesiae )

Reaction : y000773 NADH:ubiquinone oxidoreductase H+[m] + NADH[m] + ubiquinone-6[m] => NAD[m] + ubiquinol-6[m] 1.6.5.9 YALI0F25135g
should pump proton.

@edkerk
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edkerk commented Sep 18, 2020

It also has complex I:
y300029 NADH dehydrogenase (complex I) 5 H+[m] + NADH[m] + ubiquinone-6[m] => 4 H+[c] + NAD[m] + ubiquinol-6[m]
although I notice that this reaction has no gene association, probably because it's a mitochondrial gene? But y000773 seems correct?

@feiranl
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feiranl commented Sep 18, 2020

I am so sorry for not noticing this one, but then y000773 may can be deleted? since it is reported in Y. lipolytica, it should only contains the complex I not the alternative internal NADH:ubiquinone oxidoreductase.
according to "...Other yeasts and fungi, such as Yarrowia lipolytica (19), exclusively utilize Complex I for respiratory oxidation of mitochondrial NADH..."ref. The one in y000773 can only accept NADH in cytosol?

@edkerk
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edkerk commented Sep 19, 2020

If you then follow ref. 19 from that paper, they find that Yarrowia lipolytica has an external-facing NADH:ubiquinone oxidoreductase. y000773 should indeed be deleted, y000770 is the correct reaction.

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