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Fusioncatcher build fails #478

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apeltzer opened this issue Mar 15, 2024 · 6 comments
Open

Fusioncatcher build fails #478

apeltzer opened this issue Mar 15, 2024 · 6 comments
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bug Something isn't working question Further information is requested

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@apeltzer
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Description of the bug

image

Command used and terminal output

nextflow run nf-core/rnafusion -r master -profile bi,XXX (this is a singularity profile), without home mounting.

Relevant files

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System information

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@apeltzer apeltzer added the bug Something isn't working label Mar 15, 2024
@apeltzer
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@fmalmeida FYI

@rannick rannick self-assigned this Mar 15, 2024
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rannick commented Mar 15, 2024

I suspect it has to do with the home mounting. Fusioncatcher does a lot of explicit path coding that could be affected. Can you test with a standard singularity mount?

@apeltzer
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Yes, I can give it a try (y)

@rannick rannick added the question Further information is requested label Apr 3, 2024
@apeltzer
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apeltzer commented Apr 8, 2024

Hm still doesnt work I fear.
image

a.) STARfusion build error:
nhmmscan --noali --cut_ga --dfamtblout /tmp/BRphdvSPd7 --cpu=24 homo_sapiens_dfam.hmm ref_annot.cdsplus.fa
Error, cmd: dfamscan.pl -fastafile ref_annot.cdsplus.fa -hmmfile homo_sapiens_dfam.hmm -dfam_outfile __dfam_ref_annot.cdsplus.fa/dfam.out --masking_thresh --cpu 24 died with ret 6400 No such file or directory at /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/util/../lib/Pipeliner.pm line 186.
Pipeliner::run(Pipeliner=HASH(0x5589ff7c0788)) called at /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/util/dfam_repeat_masker.pl line 84
b.) Fusion Report
image

c.) Fusion Catcher Build: (also fails with 127 exit code, not fond -- I have to check thats the problem there still).

cc @fmalmeida FYI

@rannick
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rannick commented Apr 8, 2024

Trying to reproduce, but with dev. I manage to run fusion-report, I would assume your error there is due to a access problem. Are you providing the correct cosmic username and password (quiagen ones)?

@apeltzer
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apeltzer commented Apr 8, 2024

Trying to reproduce, but with dev. I manage to run fusion-report, I would assume your error there is due to a access problem. Are you providing the correct cosmic username and password (quiagen ones)?

Yes, and I already checked that they are accurate (and they are)... :-(

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