The software provides GUI for microscope control:
- advanced camera control (input and output triggers, acq. modes),
- light sheet generation via galvo scanning (NI DAQmx task configuration)
- stage scanning (with TTL pulses at defined spatial intervals)
- deformable mirror control (loading and applying commands from file)
- electrotunable lens control (manual offsetting the ETL power)
- streaming images into HDF5 file (Fiji/BigDataViewer flavor) at high speed (currently up to 60Hz)
- PC with Windows 7/10, x64.
- camera Hamamatsu Orca Flash4.3.
- NI DAQmx PCIe board with at least 2 AO channels for galvo and laser modulation (e.g. PCIe-6321).
- XY stage from ASI (MS-2000 controller).
- ETL Optotune EL-16-40-TC-VIS-5D-C.
- deformable mirror ImagineOptics Mirao52e.
(optional) Create and activate local environment from the command line, to keep things tidy
C:\Users\user\daoSPIM> cd microscope_control
C:\Users\user\daoSPIM> python -m venv venv
C:\Users\user\daoSPIM> venv\Scripts\activate.bat
(venv) C:\Users\user\daoSPIM>
Install dependencies
pip install --upgrade pip
pip install -r requirements.txt
Launch the program
python dao_spim_control.py
The code is still in development, but the author tried to keep it maximally modular and reusable. Each device is represented by a single file with GUI, controller and configuration code, independent from other devices. The main program file dao_spim_control.py connects these modules into a system. The GUI building is simplified by using a local library widget.py which takes care of PyQt5 low-level code.
See device_template.py for example.
The module files for these and other devices are independently available at kekse repo.
The camera control code was adapted from H.Babcock's storm-control software.