The installation and usage of Duplex tools

1. About

for duplex calling of guppy, needs the output of first round of guppy simplex calling.

pip install duplex-tools

duplex_tools pairs_from_summary /path/to/your/output/basecalled_fastq/sequencing_summary.txt /path/to/your/output/

Above step will generate pair_ids.txt.

duplex_tools filter_pairs /path/to/your/output/pair_ids.txt /path/to/your/simplex_fastq_output/basecalled_fastq

Get pair_ids_filtered.txt, it is for downstream duplex basecalling.