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Update to prodigal-gv
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mlhoggard authored Aug 25, 2024
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# APPENDIX (ex11) : Viral taxonomy prediction via *vContact2*

## 1. Predict genes via `prodigal`
## 1. Predict genes via *prodigal-gv*

Prodigal-gv is a variant of prodigal that has been modified to improve gene calling for giant viruses and viruses that use alternative genetic codes. More information is available [here](https://github.com/apcamargo/prodigal-gv).

!!! note "Note"

If you have already run DRAM-v to annotate viral genes, DRAM-v runs prodigal-gv in the background, so you can also use the genes.faa file generated by DRAM-v as input for vConTACT2 here.

!!! terminal "code"

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# Load dependencies
module purge
module load prodigal/2.6.3-GCC-11.3.0
module load prodigal-gv/2.9.0-GCC-11.3.0

# Set up working directories
cd /nesi/nobackup/nesi02659/MGSS_U/<YOUR FOLDER>/7.viruses

# Run Prodigal to predict genes
prodigal -p meta -q \
# Run Prodigal-gv to predict viral genes
prodigal-gv -p meta -q \
-i checkv_combined.fna \
-a viral_taxonomy/checkv_combined.faa
```
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