LabProtocols

Protocols for the Wet Lab:

-Handling and Disposing Biohazardous Agents

-Recipies for Bacterial Media

-qPCR quantification of 16S rRNA copies in samples

-Making an anaerobic glycerol stock

-Extracting RNA from bacteria or fecal samples

-Sanger 16S rRNA Sequencing of Isolates

-Toxin+ C. difficile quantificaiton by qPCR

-Fecal MIC Assays

-C. difficile Spore Preparation

-ERIC PCR for de-replicating isolates

-Culturing Caco2 cells

-Drop Plates for Bacterial Quantification