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start adding tests for tagore, low priotiy
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#!/usr/bin/env python3 | ||
''' | ||
tagore: a utility for illustrating human chromosomes | ||
https://github.com/jordanlab/tagore | ||
''' | ||
__author__ = ["Lavanya Rishishar", "Aroon Chande"] | ||
__copyright__ = "Copyright 2019, Applied Bioinformatics Lab" | ||
__license__ = "GPLv3" | ||
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import os | ||
import pickle | ||
import re | ||
import shutil | ||
import subprocess | ||
import sys | ||
from argparse import ArgumentParser, HelpFormatter | ||
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VERSION = '1.0.1' | ||
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COORDINATES = { | ||
"1": {"cx": 128.6, "cy": 1.5, "ht": 1654.5, "width": 118.6}, | ||
"2": {"cx": 301.4, "cy": 43.6, "ht": 1612.4, "width": 118.6}, | ||
"3": {"cx": 477.6, "cy": 341.4, "ht": 1314.7, "width": 118.6}, | ||
"4": {"cx": 655.6, "cy": 517.9, "ht": 1138.1, "width": 118.6}, | ||
"5": {"cx": 835.4, "cy": 461, "ht": 1195.1, "width": 118.6}, | ||
"6": {"cx": 1012.4, "cy": 524.2, "ht": 1131.8, "width": 118.6}, | ||
"7": {"cx": 1198.2, "cy": 608.5, "ht": 1047.5, "width": 118.6}, | ||
"8": {"cx": 1372.9, "cy": 692.8, "ht": 963.2, "width": 118.6}, | ||
"9": {"cx": 1554.5, "cy": 724.4, "ht": 931.6, "width": 118.6}, | ||
"10": {"cx": 1733.8, "cy": 766.6, "ht": 889.4, "width": 118.6}, | ||
"11": {"cx": 1911.5, "cy": 766.6, "ht": 889.4, "width": 118.6}, | ||
"12": {"cx": 2095.6, "cy": 769.7, "ht": 886.3, "width": 118.6}, | ||
"13": {"cx": 129.3, "cy": 2068.8, "ht": 766.1, "width": 118.6}, | ||
"14": {"cx": 301.6, "cy": 2121.5, "ht": 713.4, "width": 118.6}, | ||
"15": {"cx": 477.5, "cy": 2153.1, "ht": 681.8, "width": 118.6}, | ||
"16": {"cx": 656.7, "cy": 2232.2, "ht": 602.8, "width": 118.6}, | ||
"17": {"cx": 841.2, "cy": 2290.7, "ht": 544.3, "width": 118.6}, | ||
"18": {"cx": 1015.7, "cy": 2313.9, "ht": 521.1, "width": 118.6}, | ||
"19": {"cx": 1199.5, "cy": 2437.2, "ht": 397.8, "width": 118.6}, | ||
"20": {"cx": 1374.4, "cy": 2416.1, "ht": 418.9, "width": 118.6}, | ||
"21": {"cx": 1553, "cy": 2510.9, "ht": 324.1, "width": 118.6}, | ||
"22": {"cx": 1736.9, "cy": 2489.8, "ht": 345.1, "width": 118.6}, | ||
"X": {"cx": 1911, "cy": 1799.6, "ht": 1035.4, "width": 59}, | ||
} | ||
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CHROM_SIZES = { | ||
"hg37": { | ||
"1": 249250621, "2": 243199373, "3": 198022430, "4": 191154276, | ||
"5": 180915260, "6": 171115067, "7": 159138663, "8": 146364022, | ||
"9": 141213431, "10": 135534747, "11": 135006516, "12": 133851895, | ||
"13": 115169878, "14": 107349540, "15": 102531392, "16": 90354753, | ||
"17": 81195210, "18": 78077248, "19": 59128983, "20": 63025520, | ||
"21": 48129895, "22": 51304566, "X": 155270560, "Y": 59373566 | ||
}, | ||
"hg38": { | ||
"1": 248956422, "2": 242193529, "3": 198295559, "4": 190214555, | ||
"5": 181538259, "6": 170805979, "7": 159345973, "8": 145138636, | ||
"9": 138394717, "10": 133797422, "11": 135086622, "12": 133275309, | ||
"13": 114364328, "14": 107043718, "15": 101991189, "16": 90338345, | ||
"17": 83257441, "18": 80373285, "19": 58617616, "20": 64444167, | ||
"21": 46709983, "22": 50818468, "X": 156040895, "Y": 57227415 | ||
}, | ||
} | ||
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def printif(statement, condition): | ||
''' | ||
Print statements if a boolean (e.g. verbose) is true | ||
''' | ||
if condition: | ||
print(statement) | ||
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def draw(arguments): | ||
''' | ||
Create the SVG object | ||
''' | ||
polygons = "" | ||
try: | ||
input_fh = open(arguments.input, 'r') | ||
except (IOError, EOFError) as input_fh_e: | ||
print("Error opening input file!") | ||
raise input_fh_e | ||
svg_fn = f"{arguments.prefix}.svg" | ||
try: | ||
svg_fh = open(svg_fn, 'w') | ||
svg_fh.write(__head__) | ||
except (IOError, EOFError) as svg_fh_e: | ||
print("Error opening output file!") | ||
raise svg_fh_e | ||
line_num = 1 | ||
for entry in input_fh: | ||
if entry.startswith("#"): | ||
continue | ||
entry = entry.rstrip().split("\t") | ||
if len(entry) != 7: | ||
print(f"Line number {line_num} does not have 7 columns") | ||
sys.exit() | ||
chrm, start, stop, feature, size, col, chrcopy = entry | ||
chrm = chrm.replace('chr', '') | ||
start = int(start) | ||
stop = int(stop) | ||
size = float(size) | ||
feature = int(feature) | ||
chrcopy = int(chrcopy) | ||
if 0 > size > 1: | ||
print(f"Feature size, {size},on line {line_num} unclear. \ | ||
Please bound the size between 0 (0%) to 1 (100%). Defaulting to 1.") | ||
size = 1 | ||
if not re.match("^#.{6}", col): | ||
print(f"Feature color, {col}, on line {line_num} unclear. \ | ||
Please define the color in hex starting with #. Defaulting to #000000.") | ||
col = "#000000" | ||
if chrcopy not in [1, 2]: | ||
print(f"Feature chromosome copy, {chrcopy}, on line {line_num}\ | ||
unclear. Skipping...") | ||
line_num = line_num + 1 | ||
continue | ||
line_num = line_num + 1 | ||
if feature == 0: # Rectangle | ||
feat_start = start*COORDINATES[chrm]["ht"]/CHROM_SIZES[arguments.build][chrm] | ||
feat_end = stop*COORDINATES[chrm]["ht"]/CHROM_SIZES[arguments.build][chrm] | ||
width = COORDINATES[chrm]["width"]*size/2 | ||
if chrcopy == 1: | ||
x_pos = COORDINATES[chrm]["cx"] - width | ||
else: | ||
x_pos = COORDINATES[chrm]["cx"] | ||
y_pos = COORDINATES[chrm]["cy"] + feat_start | ||
height = feat_end-feat_start | ||
svg_fh.write(f"<rect x=\"{x_pos}\" y=\"{y_pos}\" fill=\"{col}\" width=\"{width}\"\ | ||
height=\"{height}\"/>" + "\n") | ||
elif feature == 1: # Circle | ||
feat_start = start*COORDINATES[chrm]["ht"]/CHROM_SIZES[arguments.build][chrm] | ||
feat_end = stop*COORDINATES[chrm]["ht"]/CHROM_SIZES[arguments.build][chrm] | ||
radius = COORDINATES[chrm]["width"]*size/4 | ||
if chrcopy == 1: | ||
x_pos = COORDINATES[chrm]["cx"] - COORDINATES[chrm]["width"]/4 | ||
else: | ||
x_pos = COORDINATES[chrm]["cx"] + COORDINATES[chrm]["width"]/4 | ||
y_pos = COORDINATES[chrm]["cy"]+(feat_start+feat_end)/2 | ||
svg_fh.write(f"<circle fill=\"{col}\" cx=\"{x_pos}\" cy=\"{y_pos}\"\ | ||
r=\"{radius}\"/>" + "\n") | ||
elif feature == 2: # Triangle | ||
feat_start = start*COORDINATES[chrm]["ht"]/CHROM_SIZES[arguments.build][chrm] | ||
feat_end = stop*COORDINATES[chrm]["ht"]/CHROM_SIZES[arguments.build][chrm] | ||
if chrcopy == 1: | ||
x_pos = COORDINATES[chrm]["cx"] - COORDINATES[chrm]["width"]/2 | ||
sx_pos = 38.2*size | ||
else: | ||
x_pos = COORDINATES[chrm]["cx"] + COORDINATES[chrm]["width"]/2 | ||
sx_pos = -38.2*size | ||
y_pos = COORDINATES[chrm]["cy"]+(feat_start+feat_end)/2 | ||
sy_pos = 21.5*size | ||
polygons += f"<polygon fill=\"{col}\" points=\"{x_pos-sx_pos},{y_pos-sy_pos} \ | ||
{x_pos},{y_pos} {x_pos-sx_pos},{y_pos+sy_pos}\"/>" + "\n" | ||
elif feature == 3: # Line | ||
y_pos1 = start*COORDINATES[chrm]["ht"]/CHROM_SIZES[arguments.build][chrm] | ||
y_pos2 = stop*COORDINATES[chrm]["ht"]/CHROM_SIZES[arguments.build][chrm] | ||
y_pos = (y_pos1+y_pos2)/2 | ||
y_pos += COORDINATES[chrm]["cy"] | ||
if chrcopy == 1: | ||
x_pos1 = COORDINATES[chrm]["cx"] - COORDINATES[chrm]["width"]/2 | ||
x_pos2 = COORDINATES[chrm]["cx"] | ||
svg_fh.write(f"<line fill=\"none\" stroke=\"{col}\" stroke-miterlimit=\"10\" \ | ||
x1=\"{x_pos1}\" y1=\"{y_pos}\" x2=\"{x_pos2}\" y2=\"{y_pos}\"/>" + "\n") | ||
else: | ||
x_pos1 = COORDINATES[chrm]["cx"] | ||
x_pos2 = COORDINATES[chrm]["cx"] + COORDINATES[chrm]["width"]/2 | ||
svg_fh.write(f"<line fill=\"none\" stroke=\"{col}\" stroke-miterlimit=\"10\" \ | ||
x1=\"{x_pos1}\" y1=\"{y_pos}\" x2=\"{x_pos2}\" y2=\"{y_pos}\"/>" + "\n") | ||
else: | ||
print(f"Feature type, {feature}, unclear. Please use either 0, 1, 2 or 3. Skipping...") | ||
continue | ||
svg_fh.write(__tail__) | ||
svg_fh.write(polygons) | ||
svg_fh.write("</svg>") | ||
svg_fh.close() | ||
printif(f"\033[92mSuccessfully created SVG\033[0m", ARGS.verbose) | ||
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if __name__ == "__main__": | ||
PARSER = ArgumentParser(prog="tagore", | ||
add_help=True, | ||
description=''' | ||
tagore: a utility for illustrating human chromosomes | ||
https://github.com/jordanlab/tagore | ||
''', | ||
formatter_class=lambda prog: HelpFormatter(prog, width=120, | ||
max_help_position=120)) | ||
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PARSER.add_argument('--version', action='version', | ||
help='Print the software version', | ||
version='tagore (version {})'.format(VERSION)) | ||
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# Input arguments | ||
PARSER.add_argument('-i', '--input', required=True, default=None, metavar='<input.bed>', | ||
help='Input BED-like file') | ||
PARSER.add_argument('-p', '--prefix', required=False, default="out", | ||
metavar='[output file prefix]', | ||
help='Output prefix [Default: "out"]') | ||
PARSER.add_argument('-b', '--build', required=False, default="hg38", | ||
metavar='[hg78/hg38]', | ||
help="Human genome build to use [Default: hg38]") | ||
PARSER.add_argument('-f', '--force', required=False, default=False, | ||
help="Overwrite output files if they exist already", | ||
action="store_true") | ||
PARSER.add_argument('-v', '--verbose', required=False, default=False, | ||
help="Display verbose output", | ||
action="store_true") | ||
ARGS, UNKARGS = PARSER.parse_known_args() | ||
if UNKARGS: | ||
print(f"\033[93mOne or more unknown arguments were supplied:\033[0m {' '.join(UNKARGS)}\n") | ||
PARSER.print_help() | ||
sys.exit() | ||
if ARGS.build not in ['hg37', 'hg38']: | ||
print(f"\033[91mBuild must be either 'hg37' or 'hg38', you supplied {ARGS.build}.\033[0m") | ||
sys.exit() | ||
if shutil.which("rsvg", mode=os.X_OK) is None: | ||
print(f"\033[91mCould not find `rsvg` in PATH.\033[0m") | ||
sys.exit() | ||
BASE_PATH = os.path.join(sys.prefix, 'lib', 'tagore-data', 'base.svg.p') | ||
try: | ||
BASE = open(BASE_PATH, 'rb') | ||
except (IOError, EOFError) as base_e: | ||
print(f"\033[91mCould not open {BASE_PATH}. Please reinstall tagore\033[0m") | ||
raise base_e | ||
__head__, __tail__ = pickle.load(BASE) | ||
printif(f"\033[94mDrawing chromosome ideogram using {ARGS.input}\033[0m", ARGS.verbose) | ||
if os.path.exists(f"{ARGS.prefix}.svg") and ARGS.force is False: | ||
print(f"\033[93m'{ARGS.prefix}.svg' already exists.\033[0m") | ||
OW = input(f"Overwrite {ARGS.prefix}.svg? [Y/n]: ") or "y" | ||
if OW.lower() != "y": | ||
print(f"\033[93m'tagore will now exit...\033[0m") | ||
sys.exit() | ||
else: | ||
print(f"\033[94mOverwriting existing file and saving to: {ARGS.prefix}.svg\033[0m") | ||
else: | ||
printif(f"\033[94mSaving to: {ARGS.prefix}.svg\033[0m", ARGS.verbose) | ||
draw(ARGS) | ||
printif(f"\033[94mConverting {ARGS.prefix}.svg -> {ARGS.prefix}.png\033[0m", ARGS.verbose) | ||
try: | ||
subprocess.check_output(f"rsvg {ARGS.prefix}.svg {ARGS.prefix}.png", shell=True) | ||
except subprocess.CalledProcessError as rsvg_e: | ||
printif(f"\033[91mFailed SVG to PNG conversion...\033[0m", ARGS.verbose) | ||
raise rsvg_e | ||
finally: | ||
printif(f"\033[92mSuccessfully converted SVG to PNG\033[0m", ARGS.verbose) |
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#!/bin/env python3 | ||
import pytest | ||
import sys | ||
from os import getcwd, path, pardir | ||
sys.path.append(path.abspath(path.join(getcwd()))) | ||
from tagore import printif | ||
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def test_printif_true(): | ||
from io import StringIO | ||
output = StringIO() | ||
sys.stdout = output | ||
printif("Test passed", True) | ||
assert output.getvalue().strip() == "Test passed" | ||
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def test_printif_false(): | ||
from io import StringIO | ||
output = StringIO() | ||
sys.stdout = output | ||
printif("Test false", False) | ||
assert output.getvalue().strip() == '' |