Jinghui Li, Dailu Guan, Michelle M. Halstead, Alma D. Islas-Trejo, Daniel E. Goszczynski, Catherine W. Ernst, Hao Cheng, Pablo Ross, Huaijun Zhou
We employed the long-read sequencing technology, Oxford Nanopore Technology (ONT), to annotate the pig transcriptome across 17 tissues from two Yorkshire littermate pigs. More than 9.8 million reads were obtained from a single ONT flow cell, and 69,781 unique transcripts at 50,108 loci were identified. Our study is published on Animal Genetics: https://doi.org/10.1111/age.13274.
The Nanopore sequencing data generated in this study can be found in the Sequence Read Archive (SRA) database of the National Center for Biotechnology Information with the identifier PRJNA671673 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA671673). The code for data analyses is shown in scripts/nanopore_ann.smk, and the transcriptom annotation can be obtained from https://github.com/liangend/Porcine_Nanopore/releases/download/GTF/filterSamples.gtf