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nictru authored Jun 18, 2024
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> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311.
## Pipeline tools
## Base pipeliines

- [panpipes](https://doi.org/10.1101/2023.03.11.532085)

> Curion F, Rich-Griffin C, Agarwal D, et al. Panpipes: a pipeline for multiomic single-cell and spatial transcriptomic data analysis. Published online December 18, 2023:2023.03.11.532085. doi:10.1101/2023.03.11.532085
- [scFlow](https://doi.org/10.1101/2021.08.16.456499)

- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
> Khozoie C, Fancy N, Marjaneh MM, Murphy AE, Matthews PM, Skene N. scFlow: A Scalable and Reproducible Analysis Pipeline for Single-Cell RNA Sequencing Data. Published online August 19, 2021:2021.08.16.456499. doi:10.1101/2021.08.16.456499
> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].
- [SIMBA](https://github.com/Mye-InfoBank/SIMBA)

> Trummer, N. et al. 2024. SIMBA (Single-cell Integration Methods pipeline for Big Atlases)
- [YASCP](https://github.com/wtsi-hgi/yascp)
> Ozols, M. et al. 2023. YASCP (Yet Another Single Cell Pipeline)
## Pipeline tools

- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)

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**nf-core/scdownstream** is a bioinformatics pipeline that can be used to process already quantified single-cell RNA-seq data. It takes a samplesheet and h5ad-, SingleCellExperiment/Seurat- or CSV files as input and performs quality control, integration, dimensionality reduction and clustering. It produces an integrated h5ad and SingleCellExperiment file and an extensive QC report.

The pipeline is based on the learnings and implementations from the following pipelines (alphabetical):

- [panpipes](https://github.com/DendrouLab/panpipes)
- [scFlow](https://combiz.github.io/scFlow/)
- [SIMBA](https://github.com/Mye-InfoBank/SIMBA)
- [YASCP](https://github.com/wtsi-hgi/yascp)

# ![nf-core/scdownstream](docs/images/metromap.png)

Not all of the steps shown in the metromap have already been implemented, as this pipeline is still under development. However, the following steps are already functional:
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## Credits

nf-core/scdownstream was originally written by Nico Trummer.
nf-core/scdownstream was originally written by [Nico Trummer](https://github.com/nictru).

<!-- TODO nf-core: If applicable, make list of people who have also contributed
We thank the following people for their extensive assistance in the development of this pipeline (alphabetical):

We thank the following people for their extensive assistance in the development of this pipeline:
-->
- [Fabiola Curion](https://github.com/bio-la)
- [Gregor Sturm](https://github.com/grst)
- [Jonathan Talbot-Martin](https://github.com/jtalbotmartin)
- [Lukas Heumos](https://github.com/zethson)
- [Matiss Ozols](https://github.com/maxozo)
- [Nathan Skene](https://github.com/NathanSkene)
- [Nurun Fancy](https://github.com/nfancy)
- [Riley Grindle](https://github.com/Riley-Grindle)
- [Ryan Seaman](https://github.com/RPSeaman)
- [Wojtek Sowinski](https://github.com/WojtekSowinski)

## Contributions and Support

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