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Supplemental data and website for Lima-Mendez, G., et al. (2015). Determinants of community structure in the global plankton interactome Science, 348(6237).

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Tara Oceans: Plankton Interactome

Supplemental data and website for Lima-Mendez, G., et al. (2015). Determinants of community structure in the global plankton interactome Science, 348(6237).

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1. Random Forest Results

Random forest results

  • Description:
    • Charts A, B, and C compare the NMSE (Normalized Mean Square Error) distributions of real and randomized data.
    • Chart D plots the distribution of paired differences between NMSEs obtained from the environmental (ENV) and original abundance (OTU) data (blue) versus ENV and randomized OTU data (gray).
    • Chart E shows the AUC (Area under the curve) values of the binary classifiers obtained by thresholding the output of the regression model.
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2. Variation Partitioning Plot

Variation partitioning plot

  • Description:
    • Variation partitioning of the effects of abiotic, geography, and their combined effect on plankton communities (fraction and depth wise).
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3. Overview on the Taxon Network and Its Environmentally Influenced Edges

Overview on the taxon network and its environmentally influenced edges

  • Description:
    • Displays the 51 modules resulting from leading Eigenvector clustering of the positively correlated taxon network.
    • Flow chart summarizing taxon edge numbers for different analyses.
    • Venn diagram depicting the overlap between taxon edges that are part of environmental triplets, ocean-driven taxon edges, and taxon edges classified as indirect.
    • Bar chart showing the ratio between node percentages on phylum level in the 11,043 environmentally driven taxon edges versus the remaining 81,590 taxon edges.
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4. Fraction- and Layer-wise Circos Plots

Fraction- and layer-wise circos plots

  • Description:
    • Circos plot showing order-level copresences and mutual exclusions in individual fractions and layers.
    • Band size for each taxon is proportional to the number of taxon edges, and ribbon width is proportional to the numbers of copresence/mutual exclusions within and between taxa.
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5. Examples of Ocean-specific Correlated Profiles

Examples of ocean-specific correlated profiles

  • Description:
    • Examples of edges showing global copresence and mutual exclusion patterns.
    • Ocean specific edges for Mediterranean Sea, South Pacific Ocean, Indian Ocean, and Red Sea.
    • Southern Ocean has a distinct profile of region-specific edges dominated by bacterial taxa.
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6. Potential Novel Hosts and/or Parasites

Potential novel hosts and/or parasites

  • Description:
    • Parasites in large fractions may co-occur with their hosts or with unknown parasites.
    • Distribution showing the taxonomic breakdown of the partners of parasites in the large fractions.
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7. Annotated Contigs for Novel Phages

Annotated contigs for novel phages

  • Description:
    • Annotated genome contigs of putative novel bacteriophages lacking viral representatives in the RefSeq database.
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8. Analysis of Nestedness and Modularity

Analysis of nestedness and modularity

  • Description:
    • Bacteriophage-host adjacency matrix re-arranged to visualize modularity and nestedness.
    • The adjacency matrix consists of positive associations between 816 bacteriophages and 451 hosts predicted for the surface and deep chlorophyll maximum layers.
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9. Acoel Flatworm Microscopy Images and Alignment of V9 Sequence

Acoel flatworm microscopy images and alignment of V9 sequence

  • Description:
    • Confocal laser scanning microscope images of the acoel flatworm containing several microalgal cells intracellularly.
    • Sequence alignment of the V9 Tara-oceans metabarcode of the acoel flatworm from the predicted interaction and six V9 sequences PCR amplified from six acoel flatworms found in station 22 that host symbiotic microalgae.
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  • FASTA

Tables

Name Size Description Download
W1 98K Sample information Download
W2 809K Sample Environmental parameters Download
W3 19K Variation partitioning analysis Download
W4 2.0M Random forest results Download
W5 38K False discovery rate assessed with null models Download
W6 36K Network Properties Download
W7 9.6M The TARA ocean interactome Download
W8 1.6M Indirect edge detection results Download
W9 2.3M Reference interactions among eukaryotic plankton Download
W10 285K Phylogenetic and geographic patterns in the interactome Download
W11 65K Plankton Functional Types mapped to the interactome Download
W12 106K Putative Bacteriophage-Host interactions predicted Download
W13 61K Modularity and nestedness Download

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Supplemental data and website for Lima-Mendez, G., et al. (2015). Determinants of community structure in the global plankton interactome Science, 348(6237).

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