You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Using bwa also outputs not primary alignments. These reads are flagged by the 256 flag (https://broadinstitute.github.io/picard/explain-flags.html). Preferably you would also filter them with the readcounter tool or you want the step being described in the manual.
or update documentation in the README.md around here with a note.
The text was updated successfully, but these errors were encountered:
mmterpstra
changed the title
Filter for not primary algnment or at least documenting it?
Filter for not primary alignment or at least documenting it?
Aug 29, 2023
mmterpstra
changed the title
Filter for not primary alignment or at least documenting it?
Add filter for not primary alignment or at least document it?
Aug 29, 2023
Using bwa also outputs not primary alignments. These reads are flagged by the 256 flag (https://broadinstitute.github.io/picard/explain-flags.html). Preferably you would also filter them with the readcounter tool or you want the step being described in the manual.
There is a filter for duplicate alignment here (ideally the best place to insert it):
https://github.com/shahcompbio/hmmcopy_utils/blob/29a8d1d18dfd301600d5d91832e5fe231935058c/src/bin/readCounter.cpp#L194C1-L194C63
or update documentation in the README.md around here with a note.
The text was updated successfully, but these errors were encountered: