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QC-pipe in BioNix

This repository aims to wrap a Snakemake pipeline for generating QC metrics from sequencing data into Bionix, please refer to this repository for more about the pipeline. Some bioinformatics tools such as FastQScreen, Qualimap, Samtools_stats, and MultiQC that were used in the QC-pipe and yet to be available in BioNix's tools are wrapped.

The directory subread-wf is an example workflow that I started with, to utilise bioinformatics tools already available in BioNix.

The following are some notes regarding wrapping some of the particular tools.

FastQ-Screen

FastQ-Screen always require a config file where an aligner and a list of databases should be specified, here we are using bowtie2.

MultiQC

This stage is specified solely through a flake. During this process, the FastQC was slightly redefined such that the output format is detectable by MultiQC

BioBloom

BioBloom is a tool that was not in the original QC pipeline. In my workflow, BioBloom is included to demonstrate that it can also be compiled in MultiQC's summary report.

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