Added test case to cover user-reported bug in which a read that was s…

…upposed to be Genomic was misclassified as Intergenic

testing_suite/build_test_databases.py

@@ -89,6 +89,23 @@
     print(e)
     sys.exit("Database initialization failed on chr22 annotation")
 
+try:
+    os.system("mkdir -p scratch/multiexon_read_overlapping_monoexon_transcript")
+    subprocess.check_output(
+       ["talon_initialize_database",
+        "--f", "input_files/multiexon_read_overlapping_monoexon_transcript/HMGB1P1.gtf",
+        "--a",  "gencode_v29",
+        "--5p", "500",
+        "--3p", "300",
+        "--idprefix", "ENCODEH",
+        "--l", "300",
+        "--g",  "hg38", "--o", 
+        "scratch/multiexon_read_overlapping_monoexon_transcript/talon"])
+
+except Exception as e:
+    print(e)
+    sys.exit("Database initialization failed on HMGB1P1 annotation")
+
 # Actually perform the toy_mod run
 try:
     subprocess.check_output(

testing_suite/input_files/multiexon_read_overlapping_monoexon_transcript/HMGB1P1.gtf

@@ -0,0 +1,3 @@
+chr20	HAVANA	gene	57488392	57489027	.	-	.	gene_id "ENSG00000124097.7"; gene_type "processed_pseudogene"; gene_name "HMGB1P1"; level 1; tag "pseudo_consens"; havana_gene "OTTHUMG00000032823.3";
+chr20	HAVANA	transcript	57488392	57489027	.	-	.	gene_id "ENSG00000124097.7"; transcript_id "ENST00000522557.1"; gene_type "processed_pseudogene"; gene_name "HMGB1P1"; transcript_type "processed_pseudogene"; transcript_name "HMGB1P1-201"; level 1; transcript_support_level "NA"; ont "PGO:0000004"; tag "pseudo_consens"; tag "basic"; havana_gene "OTTHUMG00000032823.3"; havana_transcript "OTTHUMT00000079848.3";
+chr20	HAVANA	exon	57488392	57489027	.	-	.	gene_id "ENSG00000124097.7"; transcript_id "ENST00000522557.1"; gene_type "processed_pseudogene"; gene_name "HMGB1P1"; transcript_type "processed_pseudogene"; transcript_name "HMGB1P1-201"; exon_number 1; exon_id "ENSE00002091063.1"; level 1; transcript_support_level "NA"; ont "PGO:0000004"; tag "pseudo_consens"; tag "basic"; havana_gene "OTTHUMG00000032823.3"; havana_transcript "OTTHUMT00000079848.3";

testing_suite/input_files/multiexon_read_overlapping_monoexon_transcript/config.csv

@@ -0,0 +1 @@
+test,test,test,input_files/multiexon_read_overlapping_monoexon_transcript/read.sam

testing_suite/input_files/multiexon_read_overlapping_monoexon_transcript/read.sam

@@ -0,0 +1,3 @@
+@HD	VN:1.6	SO:coordinate
+@SQ	SN:chr20	LN:64444167
+m54119_190202_095143/62849336/ccs	16	chr20	57487908	60	1997M5298N74M1S	*	0	0	GCAAAAACAGTACGATTTATTTTTTTATTTTTTTAATAAAAAAATTGTATTTACTTAGAAGCATTCAGAATGTCAACAAAACAGCTGCAACTTTTTTTTTTTGCAATTACAGAGTGGTATTCAGTTAACAGAACAATTATTTCGTATAAGCTGCATCAGAGACAACTGAAGATGAAAAAACTACCATCCCCATATATAACTAATTTGTGCTGCGCACCAACAAGAACCTGCTTTAAATTTCCATGCCAATTTACAACCCCCATACTCTACCAGGCAAGGTTAGTGACTATTGAAAATACCACCAGGACAGGGCCATCTAAAGACACATTTGGTAGTGTATTAACCATACAAAAAACGACACTGTACAGTTTAAAAACAAATCTTACACAGCCTTACATTTCAATTTTTTTCTTTAAAAGGAGTGAGTTGTGTACAGGGGCGTTAAATGCTTTAAGACAAGAAAAAAAACTGCGCTAGAGCCAACTTATTCATCATCATCATCTTCTTCATCTTCTTCATCTTCCTCCTCCTCATCCTCTTCATCTTCCTCATCTTCCTCCTCTTCCTTCTTTTTCTTGCTTTTTTCAGCTTTGACAACTCCCTTTTTTGCTGCCTCAGGCTTTCCTTTAGCTTGATATGCAGCAATATCCTTTTCGTATTTTTCCTTCAGCTTCGCAGCCTTCTTTTCACCAGGCTGCTTGTCATCTGCAGCAGTGTTATTCCACATCTCTCCCAGTTTCTTCGCAACATCACCAATGGACAGGCCAGGATGTTCTCCTTTGATTTTTGGGTGATACTCAGAGCAGAACAGGAAGAAGGCCGAAGGAGGCCTCTTGGGTGCATTGGGATCCTTGAACTTCTTTTTTGTCTCCCCTTTGGGAGGGATATAGGTTTTCATTTGTCTTTCATAATGGGTCTTGTCCGCCTTTGCCATATCCTCAAATTTTCCTTTCTCTTTAGCAGACATGGTCTTCCACCTCTCTGAGCACTTGTTAGAAAACTCTGAGAAGTTGACTGAAGCATCTGAGTGCTTCTTCTTATGCTCCTCCCGACAAGTTTGCACAAAAAATGCATATGATGACATTTTGCCTCTCGGCTTCTTAGGATCTCCTTTGCCCATGTTTAGTTATTTTTCTTCAGCGAGGCACAGAGTCGCCCAGTGCCCGTACGGCTCTCACTTGCCCCGGCGCTGTCTCTATGGAGCTCAATGTACTGCAGTGGCTAACAGTACGATTTATAATAGCACTCTCCCCAAAGGAAAATCCATGACAAACTTTGATACAGATCCAACATCTGTGCAAGATGTCTACGATAGATACAAAACAATGATGAAAGAAATCAAAGAAGGCCTAAATGATTGGAGGGATACACTCTTCTGTTCACGGATTGGGAGACTCAGTATTGTTAAAGTGTAAAATTCTTTCCAAATTGATCTATACATTCATTCCCCCAAAGATCTCAGCAACATTTTCTTTTTGCATAAATTCACAAGTGGATTTGAAAATTTAGATGGAAAGGCAAAGGATTCAGAATCACCAAAAACAACTTTAGAAAAGAAGAACAAAGCTGGAGGACTTGCATTATCTGAGTTTGAGGCTGACTATACGCTGTCAAGACAGCCTGGTTCTGATGAAGGACTAGCTTGGGAAAGAATGGAGCTACAACACCCACACATGTGGCCAATACTTTTTTTTTTTTTTTTGAGACGAAGTCTCACTGTAGCCCAGGCTGGAGTGCAGTGGTGTGATCTTGGCTCACGACAACCTCCGCCTCCATGGCTCAAGCCATTCTTGTGCCTCAGCCTCTGAGGAGCAGGGACTACAGGTGTGCGCCACCACATCCAGATAATATTTTGTATTTTAGTAGAGATGAGGTTTCACCATGTTGCCCAGCGTGGTCTCAAACTCCTGAGCTCAGGCGATCTGTCCTCCTCGGCTTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGTGCCTGTCAATACATTTTAATACAGCAAGCATTCACGAACTCAGAGCCAACTGGTGTCCTCACTCCCAGGTTTTGCAAAGGTCCCTTCTCTTCCTTGCCC	*	ms:i:2008	AS:i:1984	nn:i:0	ts:A:+	tp:A:P	cm:i:613	s1:i:1944	s2:i:988	de:f:0.0053	rl:i:291	NM:i:0	MD:Z:2071	jM:B:c,2	jI:B:i,57489905,57495202	RG:Z:test

testing_suite/test_multiexon_read_overlapping_monoexon_transcript.py

@@ -0,0 +1,54 @@
+import pytest
+from talon import talon, init_refs
+import sqlite3
+import pysam
+@pytest.mark.integration
+
+class TestMultExonReadOverlapsMonoGene(object):
+
+    def test_transcript_assigned_intergenic(self):
+        """ This test covers a case reported by a user where a read overlaps
+            the ~600bp mono-exonic pseudogene HMGB1P1. The read itself has
+            2 exons, the second of which contains the small pseudogene inside.
+            earlier versions of TALON classified the read as intergenic,
+            when it was actually supposed to be genomic """
+
+        # Set up references
+        database = "scratch/multiexon_read_overlapping_monoexon_transcript/talon.db" 
+        conn = sqlite3.connect(database)
+        conn.row_factory = sqlite3.Row
+        cursor = conn.cursor()
+
+        build = "hg38"
+        talon.get_counters(database)
+        run_info = talon.init_run_info(database, build)
+        struct_collection = talon.prepare_data_structures(cursor, run_info)
+
+        # Use pysam to get the read from the SAM file
+        sam_file = "input_files/multiexon_read_overlapping_monoexon_transcript/read.sam"
+        with pysam.AlignmentFile(sam_file) as sam:
+            for entry in sam:
+                sam_record = entry
+                break
+
+        # Get read attributes
+        chrom = sam_record.reference_name
+        strand = "-" if sam_record.is_reverse else "+"
+        sam_start = sam_record.reference_start 
+        sam_end = sam_record.reference_end
+
+        # Do we get any overlap with the reference gene?
+        best_gene, match_strand = talon.search_for_overlap_with_gene(chrom, min(sam_start, sam_end),
+                                                                     max(sam_start, sam_end), strand, 
+                                                                     cursor, run_info, 
+                                                                     struct_collection.tmp_gene)
+        assert best_gene == 1
+        assert match_strand == "-"
+
+        annotation_info = talon.annotate_read(sam_record, cursor, run_info, 
+                                              struct_collection, mode = 0)
+
+        assert annotation_info['gene_ID'] == 1
+        assert annotation_info['transcript_ID'] == 2
+        assert 'genomic_transcript' in annotation_info['transcript_novelty'][0]
+